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重組人周期蛋白-C（rCCNC）
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  • 更新時間：2025-05-02
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PKA-311重組人周期蛋白-C（rCCNC） 2μg/10μg/1mg Prospec

 

 

Background :
 rCCNC （cyclin C） is a member of the cyclin family of proteins. This protein interacts with cyclin-dependent kinase 8 （CDK8） and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this protein peaks in the G1 phase of the cell cycle. It has been shown that a non-cdk8-associated cellular pool of CCNC combines with cdk3 to stimulate pRb phosphorylation at S807/811 during the G0/G1 transition, and that this phosphorylation is required for cells to exit GO efficiently. Other results have shown that a marked elevated immunoreactivity of CCNC was found both in neurons and astrocytes in Alzheimer's disease （AD）. Increased levels of CCNC RNA were detected by ribonuclease protection assay （RPA） in severe AD cases and colocalization of CCNC and its preferred binding partner, CDK8, was only observed in astrocytes but not in neurons.

 
Description :
 Recombinant CNCC is expressed in E. coli as a full-length protein fused to a proprietary 16-Kd tag （a 6xHis tag is located at the very N-terminal end）.

 
Formulation:
 Recombinant CDK8 is supplied at 0.1mg/ml in a buffer containing 25 mM HEPES, pH 7.9, 125mM KCI, 0.5% TX-100, and 50% glycerol.

 
Storage:
 
 Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Please avoid freeze-thaw cycles.

 
Application:
 Recombinant human CNCC can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.

 
Purity:
 Greater than 90% （SDS-PAGE）.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 


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